GSK3B and colorectal carcinoma: Figure 3C,D shows that MK2206 inhibits indoxyl sulfate-induced phosphorylation of GSK-3β. Since c-Myc expression increased following stimulation of HCT-116 CRC cells with indoxyl sulfate for 24 h, we subsequently analyzed its upstream molecule, β-Catenin, at the same stimulation time point. MK2206 decreased the increase in β-Catenin protein levels caused by indoxyl sulfate (Figure 3E,F). Moreover, MK2206 suppressed the increase in c-Myc protein levels induced by indoxyl sulfate (Figure 3G,H).