The study confirmed that the expression of cytokines (IL-4, IL-8, IL-12p70, IFN-γ, and TNF-α) in BMDCs treated with recombination virus was significantly up-regulated and was positively correlated with the expression of MHC-II, co-stimulatory molecules CD80/86, and TLRs on the surface of BMDCs, which suggested that the secretion of TNF-α and IL-12p70 induced by the recombinant virus may be mediated by TLRs, and the potential mechanism may be related to the expression of TLR3, TLR7, and TLR9 caused by viral infection. This evidence concerns the gene IL4 and viral infectious disease.