To further characterize these pathways, validation of the RNA-sequencing results was conducted with genes relevant to the G2/M checkpoint pathway (i.e., AURKB, HMGCR, and SQLE) and E2F target pathway (i.e., AURKB and IQGAP), either from the Cancer Hallmark or Curated gene sets associated with those pathways. Here, AURKB is linked to cancer.