Through RT-qPCR and western blotting detection, we observed that IKE-treated TIPE2−/− tumor MDSCs evidently decreased the M2 immunosuppressive signature gene (Arg1) and marker protein (ARG1), whereas they markedly increased the M1 immune stimulatory signature gene (Inos) and protein (iNOS) when compared to IKE-treated WT tumor MDSCs (Figure 3c,d and Figure S3d). This evidence concerns the gene NOS2 and neoplasm.