To investigate the role of GLA in breast cancer cells, we constructed MDA-MB-231 cell lines with stable GLA knockdown using shRNA lentivirus (Figure 5A,B) and found that GLA knockdown significantly suppressed cell proliferation in both CCK-8 and EdU assay, reduced cell migration, and promoted apoptosis (Figure 5C–F). Here, GLA is linked to breast carcinoma.