In line with this, Ram et al. [25] reported the effects of the terpenoid-based bioactive compound, Nimbolide, on gut dysbiosis in a diethylnitrosamine (DEN)-HCC murine model, showing partial restoration of gut eubiosis and prevention of microbial translocation by enhancing intestinal barrier integrity (increased TJ proteins) and alleviating inflammation, as demonstrated by the downregulation of inflammation-associated proteins (TLR4, MyD88, NF-kBp65, TNF-alpha, IL-6). Here, TLR4 is linked to hepatocellular carcinoma.