To investigate the impact of CASK on cytokine production, we measured the levels of IFN-β, IFN-α, IFN-6, TNF-α, IL-6, IL-1β and IP-10 in the culture supernatant of macrophages at 12 h post-H5N1-IAV (MOI=1) infection. The gene discussed is TNF; the disease is infection.