To further examine whether lipid metabolism was associated with RLRGs and to identify the key factor involved, we compared the mRNA levels of PYM1, CTPS1, and CLTC in HCC samples from the TCGA cohort and found that CLTC levels were significantly upregulated in clinical HCC tissues relative to the other genes (Figure 7A). This evidence concerns the gene CTPS1 and hepatocellular carcinoma.