In summary, this study offers new biomarkers for clinical diagnosis through an in-depth exploration of the mechanisms of action of C1QTNF1-AS1 and miR-34a-5p within osteosarcoma cells, reveals the metabolic characteristics of osteosarcoma, and provides new targets for treatment strategies as well as theoretical support for combination therapy. The gene discussed is C1QTNF1; the disease is osteosarcoma.