Furthermore, the mRNA and protein expression of Pim3 was significantly up-regulated when H9C2 cells were exposed to HG compared with the control group (P<0.05), while treatment with YY1 siRNA, but not scrambled siRNA, markedly attenuated these hyperglycemia-induced effects (P<0.05)(Figure 5C, 5D, 5E). This evidence concerns the gene YY1 and Hyperglycemia.