As shown in Figures 6A and 6B, the protein expression of ANP and β-MHC was significantly higher in HG-treated H9C2 cells than in the control group (P<0.05), while treatment with YY1 siRNA, but not scrambled siRNA, mitigated this hyperglycemia-induced effect (P<0.05). This evidence concerns the gene NPPA and Hyperglycemia.