The current study explored the influences of three prepared dietary supplements on cholinergic function and amyloid-β through evaluation of their impact on cholinergic neurotransmission (AChE and BuChE), gene expression of GSK3β, APP, and Akt, oxidative stress and inflammatory markers in Alzheimer’s disease rats’ models induced by injection of D-galactose and aluminum chloride as well as an in silico molecular docking study. Here, APP is linked to Alzheimer disease.