Punctate nuclear staining of MLH1 is a known staining pattern associated with the MLH1 M1 antibody used in this study,35–38 affecting as many as around 75% of tumours in other studies with the (potential) loss of MLH1 expression.42 In our study, punctate MLH1 staining was seen in each of the 16 STs with the (potential) MLH1 expression loss. The gene discussed is MLH1; the disease is neoplasm.