Even after the list of 100 genes moved to the NGS4fNBS study [26], members of the project group argued against including the following IMDs: carnitine palmitoyltransferase 2 deficiency (CPT2) (CPT2, MIM *600650, #614212, #600649, #608836, #255110), carnitine-acylcarnitine translocase deficiency (CACT) (SLC25A20, MIM *613698, #212138), and mitochondrial acetoacetyl-CoA thiolase deficiency (BKT) (ACAT1, MIM *607809, #203750). This evidence concerns the gene ACAT1 and beta-ketothiolase deficiency.