To address this goal, we isolated cluster of differentiation 31–enriched (CD31-enriched) primary mouse ECs and treated them with normal (5 mmol/L) or high glucose concentrations (20 mmol/L), a condition mimicking hyperglycemia in diabetes mellitus, for a period of 48 hours, followed by bulk RNA-sequencing analysis. The gene discussed is PECAM1; the disease is Hyperglycemia.