To investigate the regulatory role of miR-301a in pancreatic cancer cell lines SW1990 and Panc-1, synthetic miR-301a mimics and inhibitors were transfected into these cells, and the mRNA and protein expression levels of MST1 (STK4), SHH, and HHIP were analyzed. The gene discussed is HHIP; the disease is familial pancreatic carcinoma.