TNF and tuberculosis: Taking the frequencies of IFN-γ+IL-2-TNF-α+-, IFN-γ+IL-2-TNF-α–, and IFN-γ-IL-2+TNF-α–specific T cells and the proportions of IFN-γ+IL-2+TNF-α–specific T cells as the fitting diagnostic index, the AUROC of MTB-specific triple-color FluoroSpot (IFN-γ/IL-2/TNF-α) to diagnose ATB was 0.882 (95%CI: 0.829–0.935), which was significantly better than that in T-SPOT.TB (AUROC = 0.811, 95%CI: 0.753–0.870, P =0.017) (Figure 4A).