Likewise, KLS-3020, which is a recombinant oncolytic vaccinia virus containing three therapeutic transgenes including PH20, IL-12, and soluble programmed death protein 1 Fc (sPD1-Fc) has demonstrated enhanced OV penetration and immune cell infiltration in CT26.WT and B16F10 tumor models (71). The gene discussed is SPAM1; the disease is neoplasm.