Similar effects were observed with a recombinant pseudorabies virus carrying transgenes for PH20, IL-18, and IFN-γ expressing PRV (rPRV-IL-18-γ-PH20), further demonstrating the efficacy of ECM-targeting OVs in improving T cell infiltration of tumors and inducing robust systemic anti-cancer immune reactions (70). The gene discussed is SPAM1; the disease is cancer.