Another PH20-expressing OV is rPRV-IL-18-γ-PH20, which is a pseudorabies virus expressing three transgenes including interleukin 18 (IL-18), interferon-gamma (IFN-γ), and PH20, and it has shown improved efficacy in preclinical experiments in cultured cells and mouse models of pancreatic cancer (Pan02) compared to control viruses rPRV-IL-18-γ and rPRV-PH20 (70). Here, SPAM1 is linked to pancreatic neoplasm.