In two cancers (KIRC and LGG) associated with SIRT1 prognosis, GSEA analysis revealed multiple significantly enriched biological pathways (Figures 9A, B), including arachidonic acid metabolism, the Toll pathway, signaling by Hippo, keratinocyte, oxidative phosphorylation, interferon signaling, the IL12 pathway, neuroactive ligand–receptor interaction, cytokine–cytokine receptor interaction, extracellular matrix organization, the NABA core matrisome and peptide ligand-binding receptors. The gene discussed is TLR4; the disease is cancer.