To further investigate whether the observed high SIE and CPE in C6/36 cells upon BinJV or BinJ-ZIKV infection was due to the lack of a functional RNAi response, we generated Argonaute2 (Ago2) deficient Aag2 cells using CRISPR/Cas9 technology (Fig. S3). The gene discussed is AGO2; the disease is Zika virus infectious disease.