In an effort to strengthen this hypothesis and confirm that the lack of an siRNA response in C6/36 is what causes these cells to develop CPE in response to ISF infections, we created a second siRNA deficient cell line, the Ago2-deficient Aag2 cells which lack functional Ago2 protein as opposed to Dcr2 in C6/36 (Fig. S3). The gene discussed is ST13; the disease is infection.