We found an increased expression of beta-catenin in AOM rats as well as in HFD-AOM rats compared to normal dietary groups, while PB was able to downregulate its expression perhaps by inhibiting the Wnt signaling pathway and causing its degradation. The effect may be associated with the flavonoids found in PB such as hydroxychavicol, chavibetol, allylpyrocatechol, and eugenol [24,25]. The gene discussed is CTNNB1; the disease is infectious otitis media.