We tested the binding of NT4 and the inhibition of this binding by heparin, using flow cytometry in various human cancer cell lines, including PANC-1 (pancreas adenocarcinoma), TE671 (rhabdomyosarcoma), HT29 (colon adenocarcinoma), MCF-7 and MDA-MB 231 (two breast adenocarcinomas). This evidence concerns the gene NTF4 and rhabdomyosarcoma.