This study aimed to characterize the triple‐hit schizophrenia‐like model rats (Wisket) by the assessment of (1) behavioral parameters in different test conditions (reward‐based Ambitus test and HomeManner system) for a prolonged period, (2) cerebral muscarinic M1 receptor (M1R) expression, and (3) the effects of olanzapine treatment on these parameters. This evidence concerns the gene CHRM1 and schizophrenia.