In a second related experiment, in addition to external clock disruption, 8- to 12-week-old IL-10-/- and WT mice were co-housed in groups of 4–5 mice per cage for 2 weeks and subjected to 16 weeks of time-restricted feeding (TRF) with food availability restricted to the last 8 h of the dark (active) phase, in order to test the effects of this intervention on colitis, clock and gut microbiota parameters (E.3, Figure 2b). This evidence concerns the gene CLOCK and colitis.