In a second related experiment, in addition to external clock disruption, 8- to 12-week-old IL-10-/- and WT mice were co-housed in groups of 4–5 mice per cage for 2 weeks and subjected to 16 weeks of time-restricted feeding (TRF) with food availability restricted to the last 8 h of the dark (active) phase, in order to test the effects of this intervention on colitis, clock and gut microbiota parameters (E.3, Figure 2b). The gene discussed is IL10; the disease is colitis.