Since GPC3 isoforms are not detectable in Sk-Hep1 cells, it would be a good model to generate two stable cell line Sk-Hep1-v1 and Sk-Hep1-v2 that were overexpressing with GPC3 variants 1 and GPC3 variant 2, respectively, to elucidate different biological activities on regulating HCC tumorigenesis and potential anti-tumor efficacies of CAR-NK92MI cells. The gene discussed is GPC3; the disease is hepatocellular carcinoma.