To determine the recognition of CAR-NK cells to the GPC3+ HCC cells, we first evaluated the expression of GPC3 in several HCC cell lines, including HCO2, HepG2, Huh7, Huh7.5, Sk-Hep1, LH86 and Hep3B cell lines. This evidence concerns the gene GPC3 and hepatocellular carcinoma.