In the present study, compared with the single intervention of CB or AKK in IBD, it was found that the combination of these two probiotics not only significantly promoted the release of anti-inflammatory factors IL-4 and IL-10 in the serum or colonic tissue but also significantly downregulated the expression of inflammatory factors such as IL-1β, IL-6, and TNF-α in the serum or colon, indicating that the combination of CB and AKK can better promote anti-inflammatory and inhibit the inflammatory response in the colonic tissue of IBD mice. This evidence concerns the gene IL1B and inflammatory bowel disease.