An additional analysis of the original AML and individual mouse samples using a more sensitive amplicon analysis (up to 0.1% variant allele frequency, VAF) detected 4 WT1 variants – 3/4 in the original AML (0.2–3.8% VAF) and 3/4 in the PDXs, one or two variants per group (Fig. S9). This evidence concerns the gene WT1 and acute myeloid leukemia.