In the case of CRS as the reference standard model, the combination of the ICT IgG–IgM test with the Sh-TSP-2 serum test had the second highest PPV (80.65%), as well as the second highest NPV (60%) and accuracy (71.43%) only after the combination of the ICT IgG–IgM test with microscopy (Table 3). Here, CD40LG is linked to congenital rubella syndrome.