To validate the increased expression of CDC20 in aneuploid cells and its direct role in determining their sensitivity to SAC inhibition, we quantified CDC20 protein expression levels in the human colon cancer cell line HCT116 and its highly aneuploid derivatives HPT1 and HPT2, as well as in the immortalized epithelial cell line RPE1 and its aneuploid derivatives, RPT1, RPT3 and RPT4. This evidence concerns the gene ADCY10 and colonic neoplasm.