In the present study, using single-cell sequencing, we showed that PCDH17 was highly and selectively expressed in vascular endothelial cells of colon cancer tissues compared to normal vascular endothelium, and demonstrated that PCDH17 could regulate the metastatic spreading of tumor cells by disrupting the integrity of the endothelial barrier. This evidence concerns the gene PCDH17 and colonic neoplasm.