Naive (Srsf1fl/fl) or Srsf1-deficient (Cd4-Cre; Srsf1fl/fl) congenitally marked (Ly5.2 + ) OT-I CD8 + T cells were transferred into immunocompetent, nonirradiated (Ly5.1 + ) C57BL/6 hosts with Hep1-6-OVA HCC tumors (Supplementary Fig. 2e). Here, CD4 is linked to hepatocellular carcinoma.