Pseudobulk analysis of GBM‐associated genes targeted for mutation further validated our hashing methodology, with increased TERT expression observed in eGBOs compared to WT organoids and reduced expression of TP53 in PRO eGBOs and both PTEN and NF1 in MES eGBOs (Figure S2C, Supporting Information). The gene discussed is TERT; the disease is glioblastoma.