Notably, highly active regulons specific to WT organoids include TFs associated with brain patterning in developing organoids,[32] including SOX2(+), POU3F3(+), and PAX6(+) (Figure 2G; Figure S3B, Supporting Information), while TFs shown to be synergistic master regulators that promote tumorigenesis and invasion in gliomas,[33] such as STAT3(+) and C/EBPβ(+), were among the highly active regulons specific to both the PRO and MES eGBOs (Figure 2G; Figure S3B, Supporting Information). This evidence concerns the gene SOX2 and central nervous system cancer.