To further assess whether the overexpression of circPCSK6 and circPCSK6‐167aa can mitigate the oncogenic effect of the activated NF‐κB signaling pathway on ICC, we used TNF‐α as an activator and discovered that the overexpression of circPCSK6 and circPCSK6‐167aa effectively reduced the TNF‐α‐induced malignant biological behavior of tumors (Figure S9A–E, Supporting Information). This evidence concerns the gene TNF and intrahepatic cholangiocarcinoma.