The occurrence of apoptosis in RTECs has been largely demonstrated to be a vital mechanism in formation process of CaOx kidney stones.[7, 23] Of note, it was reported that CHAC1 participates in triggering apoptosis.[24, 25] We used JC‐1 assays to observe the mitochondrial function of six groups, respectively. Here, CHAC1 is linked to nephrolithiasis.