Transcription levels of Ifnα and Isg15 genes at earlier infection timepoints (2 h post-BTV infection) did not show significant differences with later timepoints (Supplementary Fig. 2), more likely due to the methodology employed, since measurement of these IFN-related genes requires a 12 h stimulation with MVA, i.e. gene expression was measured after a total of 14 h of BTV infection. This evidence concerns the gene ISG15 and infection.