For signalling studies, we also generated cells conditionally overexpressing the PROS- and cancer-associated PIK3CA H1047R mutation as a positive control for increased PI3Kα signalling, while for differentiation studies we used previously reported lines conditionally expressing PIK3R1 R649W or Y657X (Huang-Doran et al., 2016; Figure 1—figure supplement 1). This evidence concerns the gene PIK3CA and cancer.