TYR and melanoma: We found that peptides from melanoma differentiation antigens (MDAs, Supplementary Table 5), such as GTYEGLLRR and SLDDYNHLV (dopachrome tautomerase, DCT), ALDGGNKHFL, ALLAVGATK (premelanosome protein, PMEL) and ALLAGLVSL (tyrosinase, TYR) were consistently upregulated by trametinib (Figures 3E–H), which is in line with the previously described upregulation of MDAs early under MAPKi treatment in Ma-Mel-63a (52) and which is further in accordance with results published using a TMT-labeling approach (6).