To identify metabolic dependencies of cancer cells derived from the same parental tissue but induced by different defined genetic lesions, we employed cell lines derived from murine HCC driven by Akt1/c-Myc/p53−/− (Akt1myr;MycOE;Trp53–/–) or by Nras/c-Myc/p53−/− (NrasG12V;MycOE;Trp53–/–) generated by transposon-mediated gene transfer in p53-deficient mice26,27. This evidence concerns the gene TP53 and hepatocellular carcinoma.