To investigate whether the essentiality of ALDOA in liver cancer cells involves catalytic or noncatalytic functions, we made use of a shRNA sequence targeting the 3′ nontranslated part of the Aldoa mRNA (shAldoa.1280) to combine inducible gene silencing with re-expression of either wild-type (WT) or mutant forms of the enzyme from the same lentiviral vector. This evidence concerns the gene ALDOA and liver cancer.