PGAM4 and cancer: Furthermore, interrogation of genetic dependencies across 1,800 cell lines reported in the Cancer Dependency Map (depmap.org) revealed common essentiality (CE) for several glycolytic enzymes, including ALDOA, GAPDH, PGK1, PGAM4 and PKM found by CRISPR-mediated deletion; however, only ALDOA was considered to be a CE gene also after gene silencing using RNAi, a targeting strategy unlikely to result in full elimination of the enzyme and only reducing its expression (Fig. 1d).