While ASD-like symptoms are evident in some GoF models, these findings suggest that ASD symptoms may result also from loss-of-function mutations in Ctnnb1. Together, this model provides valuable insights into ASD, anxiety, and cognitive impairments associated with CTNNB1 syndrome (Dong et al. 2016), thus can be suitable for therapeutic efficacy determination. Here, CTNNB1 is linked to Down syndrome.