To further investigate the roles of IFIX and NKD2 in EMT regulation, immunohistochemical staining (IHC) was performed to assess the expression of NKD2 and EMT markers (E‐cadherin, N‐cadherin, vimentin and Snail) in the xenograft tumours (Figure 6C). This evidence concerns the gene PYHIN1 and neoplasm.