Successful engraftment of immune system cells was confirmed by flow cytometry and by the presence of tumor-infiltrating lymphocytes and tumor-associated macrophages in brain/liver metastases by tissue immunostaining for CD3, CD4, CD8, CD68, human mitochondrial marker, and CD19 immune biomarkers (Supplementary Fig. S1A; ref. 38). Here, CD8A is linked to neoplasm.