T cell medium, VV peptide B8R, chicken ovalbumin (OVA), mouse PaCa DT6606, mouse lung cancer CMT64, and mouse colon cancer MC38 cells were used to stimulate splenocytes collected on days 7 and 14 after treatment ex vivo, and IFN-γ production was assessed 72 h later. This evidence concerns the gene IFNG and malignant colon neoplasm.