In pseudovirus neutralizing assays, serum samples derived from the Group 3, homologous immunization of RV-1730 effectively blocked infection from different variants (WT D614G, Delta, Omicron BA.1 and BA.2) to HEK293-ACE2 cells at very low concentrations compared to those of other groups derived from either homologus(Group1 and 2) or heterologous(Group 4 and 5). The gene discussed is ACE2; the disease is infection.