Our study identified 15 novel CpG sites annotated to 10 unique genes, including TNXB, EPPK1, ILVBL, B3GNTL1, IL18, XRCC3, C20orf3. Hypomethylation of TNXB gene and differential expression of EPPK1 protein in the placenta has been reported to be associated with GDM [45, 46]. This evidence concerns the gene EPPK1 and gestational diabetes.