To analyze the role of ADAR1, we used three different types of ADAR1 shRNAs to knock down endogenous ADAR1 in glioma cell lines (U251, T98G, and U87) through adenoviral infection and overexpressed endogenous ADAR1 in glioma cell lines (U251, T98G and U87) using the same method. The gene discussed is ADAR; the disease is central nervous system cancer.