In detail, in leukopenic mice we observed that genes metAT (AFUA_2G13630), cdoB (AFUA_5G14410), cysAT (AFUA_1G09470), isa1 (AFUA_4G10690), and hisB (AFUA_6G04700) had higher transcript levels in vivo at early (16 h) than at mid (24 h) or late (72 h) time points after infection (Figure 3b and Fig. S2a orange boxes), suggesting that these genes may be particularly important for the initiation of infection, but may not be as relevant for the progression and maintenance of infection. The gene discussed is ISCA1; the disease is infection.