Therefore, this study explores the impact of inhibiting 20-HETE synthesis on Ang II-induced myocardial hypertrophy in H9c2 cardiomyocytes, focusing on the roles of reactive oxygen species (ROS), Ca2+-mediated signaling pathways, and the recently identified 20-HETE receptor, G-protein-coupled receptor 75 (GPR75). This evidence concerns the gene GPR75 and hypertrophy.