Gene delivery through viral vectors carries a substantial risk of infection or immune‐related side effects.[55] Therefore, we attempted to use a nanocarrier to achieve delivery of PBRM1, based on the experience gained in our nanocarrier's previous series of liver tumor studies.[56, 57, 58] Through delivery tracing assays, we confirmed that these nanocarriers could enhance the intra‐tumor distribution of plasmids via fluorescence imaging experiments. This evidence concerns the gene PBRM1 and neoplasm.