Furthermore, i.v. administration of Evans blue on day 6 of infection, 2 hours prior to euthanasia, revealed no substantial vascular leak in control mice or mice with low levels of prothrombin that were infected with LCMV clone 13 (Supplemental Figure 1; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.181063DS1). This evidence concerns the gene F2 and infection.