In addition, BIN2, CMTM8, CXCL10, HCLS1, ITGB2, PTPN2, and RFTN2 were expressed at significantly higher levels in our B16 brain-derived variants compared with the parent B16-F0 and are also upregulated in human metastatic melanomas compared with primary cutaneous tumors. This evidence concerns the gene RFTN2 and metastatic melanoma.