Comparisons were made with both the murine and human signature gene sets, and B16 cells derived from all three tissues were significantly enriched in genes associated with an epithelial to mesenchymal cell transition (EMT), a core signature of cytoskeletal proteins associated with aggressive melanoma metastases in human [59], and upregulation of the KRAS signaling pathway (Fig. 2). This evidence concerns the gene KRAS and melanoma.