CD8A and cancer: First, the EPIC algorithm was used to evaluate the immune cell infiltration level, and the subsequent analysis showed a notable negative correlation of SETD4 expression with B cell (18/32), Cancer Associated Fibroblast (CAF; 19/32), CD4 T cell (15/32), CD8 T cell (10/32), endothelial cell (21/32), macrophage (29/32), and NK cell (12/32) count in most cancer types (Figure 6A).